Foreign body responses in central nervous system mimic natural wound responses and alter biomaterial functions

Biomaterials hold promise for diverse therapeutic applications in the central nervous system (CNS). Little is known about molecular factors that determine CNS foreign body responses (FBRs) in vivo , or about how such responses influence biomaterial function. Here, we probed these factors using a platform of injectable hydrogels readily modified to present interfaces with different representative physiochemical properties to host cells. We show that biomaterial FBRs mimic specialized multicellular CNS wound responses not present in peripheral tissues, which serve to isolate damaged neural tissue and restore barrier functions. Moreover, we found that the nature and intensity of CNS FBRs are determined by definable properties. For example, cationic, anionic or nonionic interfaces with CNS cells elicit quantifiably different levels of stromal cell infiltration, inflammation, neural damage and amyloid production. The nature and intensity of FBRs significantly influenced hydrogel resorption and molecular delivery functions. These results characterize specific molecular mechanisms that drive FBRs in the CNS and have important implications for developing effective biomaterials for CNS applications

Analysis the Role Conflict of Trade Union Chairman and Its Types

With the development of the market economy, the trade union work has become increasingly complex. As a special group, trade union chairman plays multiple roles. On the one hand, the trade union chairman should safeguard the rights and interests of enterprise employees, on the other hand, as the workers of the enterprise, they have to create benefits for the enterprise, this kind of dual role trigger a trade union chairman role conflict. In this paper, through reviewing the relevant literature, from the personal angle of the trade union chairman, trade union chairman of role conflict from three dimensions divided responsibility, time, interests. Through the division of the three dimension classifying trade union chairman of role conflict, and explain the causes of different types of role conflict and the corresponding countermeasures and suggestions, so as to eliminate the role of the trade union chairman conflict, give full play to the role of the trade union chairman

Fraction Constraint in Partial Wave Analysis

To resolve the non-convex optimization problem in partial wave analysis, this paper introduces a novel approach that incorporates fraction constraints into the likelihood function. This method offers significant improvements in both the efficiency of pole searching and the reliability of resonance selection within partial wave analysis

Event Generation and Consistence Test for Physics with Sliced Wasserstein Distance

In the field of modern high-energy physics research, there is a growing emphasis on utilizing deep learning techniques to optimize event simulation, thereby expanding the statistical sample size for more accurate physical analysis. Traditional simulation methods often encounter challenges when dealing with complex physical processes and high-dimensional data distributions, resulting in slow performance. To overcome these limitations, we propose a solution based on deep learning with the sliced Wasserstein distance as the loss function. Our method shows its ability on high precision and large-scale simulations, and demonstrates its effectiveness in handling complex physical processes. By employing an advanced transformer learning architecture, we initiate the learning process from a Monte Carlo sample, and generate high-dimensional data while preserving all original distribution features. The generated data samples have passed the consistence test, that is developed to calculate the confidence of the high-dimentional distributions of the generated data samples through permutation tests. This fast simulation strategy, enabled by deep learning, holds significant potential not only for increasing sample sizes and reducing statistical uncertainties but also for applications in numerical integration, which is crucial in partial wave analysis, high-precision sample checks, and other related fields. It opens up new possibilities for improving event simulation in high-energy physics research

Cell-specific and region-specific transcriptomics in the multiple sclerosis model: Focus on astrocytes.

Changes in gene expression that occur across the central nervous system (CNS) during neurological diseases do not address the heterogeneity of cell types from one CNS region to another and are complicated by alterations in cellular composition during disease. Multiple sclerosis (MS) is multifocal by definition. Here, a cell-specific and region-specific transcriptomics approach was used to determine gene expression changes in astrocytes in the most widely used MS model, experimental autoimmune encephalomyelitis (EAE). Astrocyte-specific RNAs from various neuroanatomic regions were attained using RiboTag technology. Sequencing and bioinformatics analyses showed that EAE-induced gene expression changes differed between neuroanatomic regions when comparing astrocytes from spinal cord, cerebellum, cerebral cortex, and hippocampus. The top gene pathways that were changed in astrocytes from spinal cord during chronic EAE involved decreases in expression of cholesterol synthesis genes while immune pathway gene expression in astrocytes was increased. Optic nerve from EAE and optic chiasm from MS also showed decreased cholesterol synthesis gene expression. The potential role of cholesterol synthesized by astrocytes during EAE and MS is discussed. Together, this provides proof-of-concept that a cell-specific and region-specific gene expression approach can provide potential treatment targets in distinct neuroanatomic regions during multifocal neurological diseases

Effect of magnesium ions on the thermal stability of human poly(A)-specific ribonuclease

AbstractPoly(A)-specific ribonuclease (PARN), a member of the DEDD family, is a key enzyme involved in the deadenylation of mRNA in higher eukaryotic cells. In this research, it was found that Mg2+ could protect PARN against thermal inactivation by increasing the midpoint of inactivation and decreasing the inactivation rate. This protective effect was unique to Mg2+ in a concentration-dependent manner. However, the thermal unfolding and aggregation was promoted by the addition of Mg2+ at high temperatures. These results revealed that Mg2+ might have dual effects on PARN stability: protecting the active site but endangering the overall structural stability